Study on the effectiveness of a multi-toxin immunoaffinity clean-up for reliable cost-effective HPLC-FLD analysis of mycotoxins in corn-based foods

A different, reliable, and cost-effective strategy for the analysis of aflatoxins, ochratoxin A, zearalenone in corn-based foods was proposed, including one multi-toxin immunoaffinity column (IAC) sample preparation three different high-performance liquid chromatography fluorescence detection methods. The analytical procedures were tested verified, keeping mind their occurrence at trace levels foods. With validation proposed IAC methodology comparison performance characteristics with methods using a single-toxin IAC, we confirmed reliability procedure versus IAC. validated by revealing satisfactory characteristics; example, obtained values limit significantly lower than maximum limits all mycotoxins concern. In addition, recovery between 70.9 % 106.1 interest, precision 10.5 %.